China's oats are mostly bare oats, and the main planting areas are in high-altitude regions such as northwest, north China, and southwest, and these areas are mostly densely distributed saline-alkali lands in China. The seed germination (germination potential, germination rate) and seedling growth (root length, seedling height) were used to evaluate the salt tolerance of naked oat varieties at the germination stage. A comprehensive assessment of the salt tolerance of oat germplasm was established by weighted membership function method. The technology system comprehensively evaluates the bare oat germplasm materials from home and abroad in order to screen excellent germplasms with strong salt tolerance during the germination period and provide parental materials for the salt-tolerant breeding of naked oats, so as to exploit and improve naked oats. The positive role of saline-alkali land.
The artificial salt climate incubator was used to identify the salt tolerance of naked oats at the germination stage. The sprouting method on the petri dish was used to conduct salt stress with 1.2% NaCl aqueous solution (formulated from chemical grade NaCl and distilled water). The test method was based on Wu Junying. And other methods have changed slightly. Each species should be filled with seeds that are full of no defects and are basically the same size, soaked in 5% sodium hypochlorite aqueous solution for 15 minutes for disinfection, then rinsed with tap water for 2 minutes, and then washed with distilled water for 1 minute. Spread on gauze and dry naturally. Two treatments of distilled water control and salt stress were set for each variety. Each treatment was repeated three times. One dish (Φ9cm) was repeated one time, and three sheets of neutral filter paper (Φ10cm) were plated in each dish. Using a pipette, 5 mL of 1.2% aqueous NaCl solution was added to a salt-stressed Petri dish, 5 mL of distilled water was added to the control, and the dried seeds were evenly placed in a Petri dish with wolfberry (70% alcohol). Pick 50 grains per dish and close the lid. Finally, the culture dish was placed in an artificial climate incubator together. The culture conditions were a constant temperature of 25°C, relative humidity (70±5)%, light for 12 hours per day (6:00-18:00), and light intensity of 150 μmol·m-2·s. -1. Keep the filter paper saturated with water and supplement the loss of moisture with distilled water.
The radicle was at least equal to the seed length, and the seedling height was not shorter than 1/2 of the seed length as the germination standard. On the fourth day of culture (about 96 hours of cultivation), the number of germinations of each variety was counted, and the germination potential of each variety was calculated; on the seventh day of culture (about 168 hours of cultivation), the number of germinations of each variety was firstly counted, the germination rate was calculated, and then Ten seedlings were randomly picked from the two treatments and the longest root length (the length from the seed embryo to the longest root apex, hereinafter referred to as "root length") and the seedling height (from the seed embryo to the tip of the leaf were measured. length). There was no significant difference in germination power, germination rate, root length, and seedling height between the replicates, so the average value of each index was taken.
The use of artificial climate incubators for cultivation studies found that the germination rate can be used as an indicator for the rapid identification of salt tolerance or initial screening of a large number of materials during the germination period of naked oats. Based on the comprehensive evaluation and cluster analysis of the weighted membership function method, 278 raw oat germplasm materials can be classified into 5 salt-tolerant grades: 17 materials with high salt tolerance (Grade 1) and 114 samples with salt tolerance (Grade 2). Materials, moderately salt-tolerant (Grade 3) 106 materials, sensitive (Grade 4) 25 materials, and high-sensitivity (Grade 5) 16 materials.
The artificial salt climate incubator was used to identify the salt tolerance of naked oats at the germination stage. The sprouting method on the petri dish was used to conduct salt stress with 1.2% NaCl aqueous solution (formulated from chemical grade NaCl and distilled water). The test method was based on Wu Junying. And other methods have changed slightly. Each species should be filled with seeds that are full of no defects and are basically the same size, soaked in 5% sodium hypochlorite aqueous solution for 15 minutes for disinfection, then rinsed with tap water for 2 minutes, and then washed with distilled water for 1 minute. Spread on gauze and dry naturally. Two treatments of distilled water control and salt stress were set for each variety. Each treatment was repeated three times. One dish (Φ9cm) was repeated one time, and three sheets of neutral filter paper (Φ10cm) were plated in each dish. Using a pipette, 5 mL of 1.2% aqueous NaCl solution was added to a salt-stressed Petri dish, 5 mL of distilled water was added to the control, and the dried seeds were evenly placed in a Petri dish with wolfberry (70% alcohol). Pick 50 grains per dish and close the lid. Finally, the culture dish was placed in an artificial climate incubator together. The culture conditions were a constant temperature of 25°C, relative humidity (70±5)%, light for 12 hours per day (6:00-18:00), and light intensity of 150 μmol·m-2·s. -1. Keep the filter paper saturated with water and supplement the loss of moisture with distilled water.
The radicle was at least equal to the seed length, and the seedling height was not shorter than 1/2 of the seed length as the germination standard. On the fourth day of culture (about 96 hours of cultivation), the number of germinations of each variety was counted, and the germination potential of each variety was calculated; on the seventh day of culture (about 168 hours of cultivation), the number of germinations of each variety was firstly counted, the germination rate was calculated, and then Ten seedlings were randomly picked from the two treatments and the longest root length (the length from the seed embryo to the longest root apex, hereinafter referred to as "root length") and the seedling height (from the seed embryo to the tip of the leaf were measured. length). There was no significant difference in germination power, germination rate, root length, and seedling height between the replicates, so the average value of each index was taken.
The use of artificial climate incubators for cultivation studies found that the germination rate can be used as an indicator for the rapid identification of salt tolerance or initial screening of a large number of materials during the germination period of naked oats. Based on the comprehensive evaluation and cluster analysis of the weighted membership function method, 278 raw oat germplasm materials can be classified into 5 salt-tolerant grades: 17 materials with high salt tolerance (Grade 1) and 114 samples with salt tolerance (Grade 2). Materials, moderately salt-tolerant (Grade 3) 106 materials, sensitive (Grade 4) 25 materials, and high-sensitivity (Grade 5) 16 materials.
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